OlivetoStefania INGM, Policlinico Milano, PAD INVERNIZZI & University of MilanItaly

My research focuses on investigating the molecular mechanisms that regulate tumor development in malignant pleural mesothelioma. As the basis of my research, I applied numerous cellular and molecular biology approaches. My research line has developed in two distinct phases In the first phase of my career, I concentrated on studying translational control and its regulation in malignant pleural mesothelioma, focusing on two distinct areas: 1. Characterization of the translation initiation factor eIF6, both in tumor-derived cell lines and in fresh surgical tumor material 2. Characterization of microRNAs associated with the protein synthesis apparatus, which directly regulate gene expression and translation. In the second phase of my career, I investigated translational control in the tumor microenvironment, focusing my research on the translational apparatus of tumor-infiltrating lymphocytes. In particular, I developed an innovative technique that allows single-cell analysis of the translatome of regulatory T cells and their molecular characterization. During this phase, I also conducted analyses of lymphocytic cells in various types of tumors. I'm also actively working on developing a fluidic system for scoring the viability and tumorigenicity of gene-edited hematopoietic stem cells and developing new static and dynamic 3D cellular models.